BioImageXD

Free open source software for analysis and visualization of multidimensional biomedical images

Features

Supported file formats

  • Carl Zeiss LSM
  • Olympus FV1000 OIF
  • Leica SP2 TXT and SP5 LIF
  • OME-TIFF (read & write)
  • MRC
  • Multipage TIFF and single/series of TIFF, PNG and JPEG images (read & write)
  • VTK XML (write)
  • BXD & BXC internal formats

Image viewing modes (with time series support)

  • Slices
  • Gallery
  • Orthographic sections
  • Maximum and average intensity projection
  • 3D rendering

3D modules

  • Software ray casting and OpenGL texture mapping
  • Surface rendering
  • Ortographic sections
  • Visualization of motion tracks
  • Clipping with plane and box
  • Warping
  • PDB visualization
  • Axes, angle and distance measurements

Animator

  • Create your own videos with the Animator
  • Camera path editing
  • Keyframes
  • 4D support

Batch processor

  • Support for multiple parallel processing pipelines
  • Support almost any operation in BioImageXD
  • Produces result images and aggregates selected result statistics into spreadsheets for further processing

Image processing

  • Color, brightness, contrast and gamma adjustment
  • Noise filtering
  • Registration methods
  • Deconvolution
  • Fluorescence bleach correction
  • Color and intensity transfer function adjustments

Quantitative analysis

  • Colocalization analysis (voxel and object)
  • 3D segmentation methods
  • Object analysis
  • Motion tracking
  • Internalization analysis
  • ROI analysis
  • FRAP analysis
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