Free open source software for analysis and visualization of multidimensional biomedical images


The latest and older versions of the software are always available at the SourceForge page. The bug tracker can also be found there, please use it. The latest packages of the software are:


Windows 64-bit binary installer
Download BioImageXD 1.0 (r1799)


Windows 32-bit binary installer
Download BioImageXD 1.0 (r1799)


Linux 64-bit package
Download BioImageXD 1.0 (r1799)


Mac OS X 10.6/10.7 64-bit package
Download BioImageXD 1.0 (r1799)


Installation instructions, Windows


Windows packages are distributed as installers. Just run the installer and follow coming instructions to install BioImageXD to your system. You might need system administrator rights depending on your system settings.


Installation instructions, Linux


Linux package is not yet distributed as installer and it has prerequisite of Python 2.6/2.7 and wxPython 2.8.x installed in the system. To install BioImageXD on Linux, download the Linux package and extract it to the directory where you want to install BioImageXD. Then follow instructions on readme_linux.txt to set LD_LIBRARY_PATH. After setting that environment variable, you are ready to run BioImageXD using command python


Installation instructions, Mac OSX


Mac OSX package is not yet distributed as disk image, we are working on it. Also the Mac OSX package currently uses different version of wx GUI library than Windows or Linux packages, so there can be some GUI related bugs in OSX version. To install and use BioImageXD in Mac OSX, you need to have at least a basic knowledge of shell ( Mac OSX 10.6/10.7 package requires Python 2.7 installed in the system, everything else is included in the package. After you have downloaded the BioImageXD package and extracted it (in terminal use command tar xzf BXD_package_name.tar.gz), read file readme_osx.txt for instructions to set DYLD_LIBRARY_PATH. If you have extracted BioImageXD to /Users/username/BioImageXD then for instance you only need to set the following two lines to file /Users/username/.bash_profile:


export DYLD_LIBRARY_PATH=$DYLD_LIBRARY_PATH:${BXD_PATH}/Libraries/:${BXD_PATH}/Libraries/python2.7/site-packages/vtkbxd/:${BXD_PATH}/Libraries/vtk-5.6/:${BXD_PATH}/Libraries/InsightToolkit/


After that BioImageXD can be launched in the BioImageXD directory using command python2.7 in every new terminal application.


Source code
You can access the source code through the SourceForge subversion service.
The repository can be accessed at the following URL:


Sample data
Sample data can be downloaded from SourceForge. Sample data package includes one 3D and one 4D LSM stack of two channels.


Getting started guide is available in the software (Help->Help) and in installation subdirectory Help. Gettting started guide is also available from SourceForge.

Webinar: Easy visualization and analysis with BioImageXD
Teaches you how to use BioImageXD in practise:

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